https://referensmetodik.folkhalsomyndigheten.se/index.php?action=history&feed=atom&title=Bilaga_2_%28CNS%29 2026-05-14T11:43:53Z Versionshistorik för denna sida på wikin MediaWiki 1.35.8 https://referensmetodik.folkhalsomyndigheten.se/index.php?title=Bilaga_2_(CNS)&diff=4722&oldid=prev 2009-12-19T11:46:38Z

<p><span dir="auto"><span class="autocomment">Detektion av bakterier i direktutstryk: Akridinorangefärgning</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="sv"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Äldre version</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Versionen från 19 december 2009 kl. 11.46</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l31" >Rad 31:</td> <td colspan="2" class="diff-lineno">Rad 31:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Undersök utstryket i fluorescensmikroskop.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Undersök utstryket i fluorescensmikroskop.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Detektionsgränsen anges till <del class="diffchange diffchange-inline">i </del>&gt;=&lt;math&gt;10^4&lt;/math&gt; CFU/mL. Avläsning kan ske vid 400x förstoring.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Detektionsgränsen anges till &gt;=&lt;math&gt;10^4&lt;/math&gt; CFU/mL. Avläsning kan ske vid 400x förstoring.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Kategori: CNS-infektioner]]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Kategori: CNS-infektioner]]</div></td></tr> </table>

Magnus Thore https://referensmetodik.folkhalsomyndigheten.se/index.php?title=Bilaga_2_(CNS)&diff=4526&oldid=prev 2009-12-16T15:10:56Z

<p><span dir="auto"><span class="autocomment">Detektion av bakterier i direktutstryk: Akridinorangefärgning</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="sv"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Äldre version</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Versionen från 16 december 2009 kl. 15.10</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l15" >Rad 15:</td> <td colspan="2" class="diff-lineno">Rad 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*&#039;&#039;Akridinorange, brukslösning&#039;&#039;</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*&#039;&#039;Akridinorange, brukslösning&#039;&#039;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**Akridinorange 5 mg</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>**Akridinorange 5 mg</div></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>**Acetatbuffert 0,2 <del class="diffchange diffchange-inline">molfL pil </del>4,0 <del class="diffchange diffchange-inline">10OmL</del></div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>**Acetatbuffert 0,2 <ins class="diffchange diffchange-inline">mol/L ph </ins>4,0 <ins class="diffchange diffchange-inline">100mL</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>VARNING! Akridinorange är cancerframkallande. Använd handskar!</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>VARNING! Akridinorange är cancerframkallande. Använd handskar!</div></td></tr> </table>

Magnus Thore https://referensmetodik.folkhalsomyndigheten.se/index.php?title=Bilaga_2_(CNS)&diff=4525&oldid=prev 2009-12-16T15:10:30Z

<p></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="sv"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Äldre version</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Versionen från 16 december 2009 kl. 15.10</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l31" >Rad 31:</td> <td colspan="2" class="diff-lineno">Rad 31:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Undersök utstryket i fluorescensmikroskop.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Undersök utstryket i fluorescensmikroskop.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Detektionsgränsen anges till i &gt;=<del class="diffchange diffchange-inline">10e4 </del>CFU/mL. Avläsning kan ske vid 400x förstoring.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Detektionsgränsen anges till i &gt;=<ins class="diffchange diffchange-inline">&lt;math&gt;10^4&lt;/math&gt; </ins>CFU/mL. Avläsning kan ske vid 400x förstoring.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Kategori: CNS-infektioner]]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Kategori: CNS-infektioner]]</div></td></tr> </table>

Magnus Thore https://referensmetodik.folkhalsomyndigheten.se/index.php?title=Bilaga_2_(CNS)&diff=2724&oldid=prev 2009-11-01T07:12:44Z

<p>Skapade sidan med &#039;&#039;&#039;Till innehållsförteckningen för <a href="/w/Referensmetodik:_Infektioner_i_centrala_nervsystemet" title="Referensmetodik: Infektioner i centrala nervsystemet">Referensmetodik: Infektioner i centrala nervsystemet</a>&#039;&#039; ---- == Bilaga 2 == === Detektion av bakterier i direktutstryk: Akridinorang…&#039;</p> <p><b>Ny sida</b></p><div>&#039;&#039;Till innehållsförteckningen för [[Referensmetodik: Infektioner i centrala nervsystemet]]&#039;&#039;<br /> <br /> ----<br /> <br /> <br /> == Bilaga 2 ==<br /> <br /> <br /> <br /> === Detektion av bakterier i direktutstryk: Akridinorangefärgning ===<br /> <br /> <br /> &#039;&#039;&#039;Reagens&#039;&#039;&#039;<br /> <br /> *&#039;&#039;Akridinorange, brukslösning&#039;&#039;<br /> **Akridinorange 5 mg<br /> **Acetatbuffert 0,2 molfL pil 4,0 10OmL<br /> <br /> VARNING! Akridinorange är cancerframkallande. Använd handskar!<br /> <br /> &#039;&#039;&#039;Fixering av utstryk&#039;&#039;&#039;<br /> * Lufttorka utstryket på en plan yta.<br /> * Fixera över låga.<br /> * Placera alternativt några droppar metanol på objektglaset. Låt ligga 2 min. Låt kvarvarande metanol rinna av utan att skölja och låt utstryket lufttorka igen.<br /> <br /> &#039;&#039;&#039;Färgningsprocedur&#039;&#039;&#039;<br /> * Flöda det fixerade utstryket med akridinorangelösning.<br /> * Låt ligga 2 min.<br /> * Skölj med kranvatten.<br /> * Torka objektglaset.<br /> * Undersök utstryket i fluorescensmikroskop.<br /> <br /> Detektionsgränsen anges till i &gt;=10e4 CFU/mL. Avläsning kan ske vid 400x förstoring.<br /> <br /> [[Kategori: CNS-infektioner]]</div>

Magnus Thore