Ded isolates we previously isolated from strawberry plants in the Czech

The Ve exchanges increases (their frequency in-Appl. Sci. 2021, 11,15 ofcreases), the worth of sequences with the ITS region employed in identification were deposited into NCBI GenBank (Table 1). The frequently employed fungicide Aliette was also incorporated amongst the variants; the seedlings have been immersed inside a 1 suspension for 20 min just prior to planting. Clean water was employed in control variants rather than the inoculum from the pathogen or BCAs. Frigo plants on the strawberry cultivars Sonata, Wendy, and Alue 0.05 by the Whitney U test. AOH = alternariol; AME = alternariol monomethyl Karmen had been planted in each and every plant pot a single week after the BCAs; the seedlings were stored at 5 C for one particular week just before planting. In the course of the cultivation, the plants have been arranged randomly, separately in pathogenic and nonpathogenic variants to precisely avoid the transfer of pathogen to nonpathogenic variants. The plants had been then cultivated within a greenhouse or cultivating room inside a 12 h/12 h photoperiod regime for eight weeks. Immediately after cultivation, all plants in every variant were removed in the pots, the substrate was carefully washed out, and the plants have been dissected. Their number along with the fresh weight of leaves, fruits, and blooms; the diameter and weight in the rhizome; as well as the length and weight of your roots had been recorded.Agriculture 2021, 11,four ofTable 1. Microorganisms utilised in experiment.BCA Gliorex Clonoplus \ Contans Hirundo Prometheus \ Polyversum Integral Pro Xilon IDs of Phytophthora cactorum Isolates Made use of 17_04_12 18_10_14a 17_12_20 17_15_10b 17_23_19 19_28_2 19_28_10 17_30_18 17_34_7 17_45_1b Active Compound Trichoderma sp., Clonostachys sp. Clonostachys rosea Clonostachys rosea isolate 156 Coniothyrium minitans Bacillus sp. Pseudomonas Pseudomonas sp., isolate Pythium oligandrum Bacillus amyloliquefaciens (MBI 600) Trichoderma asperellum kmen T34 NCBI GenBank Accession Numbers MW193106 MW193116 MW193108 OK448179 MW193104 OK257676 OK257674 OK257655 OK257657 OK257664 Dilution (g/mL) two 2 \ 0.two 0.05 0.05 \ 0.15 0.05 0.5 No of Spores (cells)/Pot 1.7 mil 0.3 mil 0.three mil 3.three mil 0.eight mil 0.8 mil 0.eight mil 2500 18.3 mil 83,Locality of Origin Kunratice/Central Bohemia B ezany II/Central Bohemia r Plzen/West Bohemia Holesov/South Moravia Lomec/West Bohemia P elovice/East Bohemia r P elovice/East Bohemia r Sv ov/North Bohemia Lysnad Labem/Central Bohemia Veselu Semil/East BohemiaFor BCA preparations, the total dilution utilised in water as well as the resulting number of propagules per cultivating pot for treatment of every single plant are given.Ded isolates we previously isolated from strawberry plants within the Czech Republic (Table 1). The species identity of isolates was determined making use of the metabarcoding of the ITS region of rDNA following morphological determination based on the shape of reproductive structures. The sequences from the ITS area made use of in identification were deposited into NCBI GenBank (Table 1). All isolates had been deposited inside the Collection of Agriculturally Vital Fungi (VURV-F). Those isolates were cultivated in 1000 mL Erlenmeyer flasks in V8 broth for 1 week at 22 C. After cultivation, the whole content material with the flasks was filtered via sterile cheesecloth, and the mycelium was homogenized with an Ultra-Turrax homogenizer (15,000 rpm) in autoclaved demineralized water. An inoculum prepared within this way was used in the inoculation in the substrate within the plant pots.